Journal: Pharmaceutics

Article Title: Material Characterisation and Stratification of Conjunctival Epithelial Cells on Electrospun Poly(ε-Caprolactone) Fibres Loaded with Decellularised Tissue Matrices

doi: 10.3390/pharmaceutics13030318

Figure Lengend Snippet: Experimental set-up for in vitro culture of human conjunctival epithelial cells demonstrating preparation of transwells with removal of polyester (PET) membrane and replacement with electrospun scaffolds. Cells were seeded and cultured fully submerged for two weeks with keratinocyte serum free media (KSF-media), followed by one week with stratification media and a further week at the air/liquid interface with media received on the cells’ basal side. Created with BioRender.com.

Article Snippet: A human conjunctival epithelial cell (HCjE) line [ ] was cultured at 37 °C and 5% CO 2 in 75 cm 2 sterile flasks (Greiner Bio-one, Stonehouse, UK) with keratinocyte serum-free medium (KSFM; Thermo Fisher, Loughborough, UK) supplemented with 25 μg/mL bovine pituitary extract (Thermo Fisher), 0.4 mM calcium chloride (Merck, Gillingham, UK), 0.2 ng/mL recombinant human epidermal growth factor (rEGF, Thermo Fisher, Loughborough, UK), 100 U penicillin and 0.1 mg/mL streptomycin (Merck, Gillingham, UK) and 2.5 μg/mL amphotericin B (Merck, Gillingham, UK).

Techniques: In Vitro, Membrane, Cell Culture

Journal: Pharmaceutics

Article Title: Material Characterisation and Stratification of Conjunctival Epithelial Cells on Electrospun Poly(ε-Caprolactone) Fibres Loaded with Decellularised Tissue Matrices

doi: 10.3390/pharmaceutics13030318

Figure Lengend Snippet: Representative scanning electron microscopy images of human conjunctival epithelial cells cultured on electrospun scaffolds fabricated from poly(ε-caprolactone) (PCL) and PCL with addition of 1% or 10% decellularised tissue powder (small intestinal submucosa (SIS) and urinary bladder matrix (UBM)). Images: top row = ×1000 (scale = 50 μm), middle row = ×5000 (scale = 10 μm), bottom row = ×10,000 (scale = 5 μm); arrows indicate presence of microvilli.

Article Snippet: A human conjunctival epithelial cell (HCjE) line [ ] was cultured at 37 °C and 5% CO 2 in 75 cm 2 sterile flasks (Greiner Bio-one, Stonehouse, UK) with keratinocyte serum-free medium (KSFM; Thermo Fisher, Loughborough, UK) supplemented with 25 μg/mL bovine pituitary extract (Thermo Fisher), 0.4 mM calcium chloride (Merck, Gillingham, UK), 0.2 ng/mL recombinant human epidermal growth factor (rEGF, Thermo Fisher, Loughborough, UK), 100 U penicillin and 0.1 mg/mL streptomycin (Merck, Gillingham, UK) and 2.5 μg/mL amphotericin B (Merck, Gillingham, UK).

Techniques: Electron Microscopy, Cell Culture

Journal: Pharmaceutics

Article Title: Material Characterisation and Stratification of Conjunctival Epithelial Cells on Electrospun Poly(ε-Caprolactone) Fibres Loaded with Decellularised Tissue Matrices

doi: 10.3390/pharmaceutics13030318

Figure Lengend Snippet: ( A ) Representative confocal images of DAPI-stained human conjunctival epithelial cell nuclei cultured on electrospun scaffolds fabricated from poly(ε-caprolactone) (PCL) and PCL with addition of 1% or 10% decellularised tissue powder (small intestinal submucosa (SIS) and urinary bladder matrix (UBM)). Images shown; XY z-stack (scale = 50 μm), XZ side view and YZ side view. ( B ) Cell layers observed from 6 set regions of interest presented as percentages within each group ( n = 3). ( C ) Total number of cell nuclei counted within 6 regions of interest for each group ( n = 3). One-way ANOVA with Tukey’s multiple comparisons, significance for p < 0.05. Statistical differences shown by p values.

Article Snippet: A human conjunctival epithelial cell (HCjE) line [ ] was cultured at 37 °C and 5% CO 2 in 75 cm 2 sterile flasks (Greiner Bio-one, Stonehouse, UK) with keratinocyte serum-free medium (KSFM; Thermo Fisher, Loughborough, UK) supplemented with 25 μg/mL bovine pituitary extract (Thermo Fisher), 0.4 mM calcium chloride (Merck, Gillingham, UK), 0.2 ng/mL recombinant human epidermal growth factor (rEGF, Thermo Fisher, Loughborough, UK), 100 U penicillin and 0.1 mg/mL streptomycin (Merck, Gillingham, UK) and 2.5 μg/mL amphotericin B (Merck, Gillingham, UK).

Techniques: Staining, Cell Culture